Table 1.
Sequences of primers used.
| Gene | Primer name | 5′to 3′ sequence | Notes |
|---|---|---|---|
| Gene deletion | |||
| Mrmep1 | Mrmep1-5F | AACTGCAGTAGCAAGCATAGCCAGTC | PCR identification of Mrmep1 |
| Mrmep1-5R | AACTGCAGAGTGGCCTTTACTTTGAG | Deletion transformants | |
| Mrmep1-3F | GGACTAGTTCGATTTAGTGGGAATGAGC | ||
| Mrmep1-3R | GCTCTAGACATCGCTTGAATCTCCTGTG | ||
| Mrmep1-F | ACAACCAGATGGACGTGCTAAAC | ||
| Mrmep1-R | TAACTGTTGGCAAAGTCCACCTC | ||
| Mrmep2 | Mrmep2-5F | AACTGCAGCGAGTTTGGGAAAGTAGTGC | PCR identification of MrMEP2 |
| Mrmep2-5R | AACTGCAGCAGACGGCTGGTTGTAAGA | Deletion transformants | |
| Mrmep2-3F | GACTAGTGTCTTCCGATGGCTGCTT | ||
| Mrmep2-3R | GCTCTAGACATGCCTTGGTTGTCTGC | ||
| Mrmep2-F | AGGCACTCCAACCAATTAGCA | ||
| Mrmep2-R | AAGCAGCCATCGGAAGACAAGG | ||
| bar | bar-F | TCGTCAACCACTACATCGAGAC | |
| bar-R | GAAGTCCAGCTGCCAGAAAAC | ||
| ben | ben-F | GGTAACTCCACCGCCATCCA | |
| ben-R | GCAGGGTATTGCCTTTGGACTT | ||
| Gene complementation | |||
| Mrmep1 | cMrmep1-5F | CACGCCTGTCGTATTCCAGCATT | |
| cMrmep1-3R | TGTTCTTGATGTTGTGCTCGCCC | ||
| Mrmep2 | cMrmep2-5F | CCGCTCGAGCGCCGCTCTTTACGTTCTTT | |
| cMrmep2-3R | AACTGCAGTTACTCGTAGGAATACCCCATG | ||
| Southern blotting | |||
| S-bar | GCCGTGCCACCGAGGCGGACATGCCGGCGGTCTGCACCATCGTCAACCACTACATCGAG | ||
| S-ben | CCCCTTCTGTGCCTCTACCTACTGCTGCCCGACTCATTATGATCCTGCTCGCTTTCTCG | ||
| Gene expression analysis and RT-PCR identification of deletion transformants | |||
| Mrmep1 | Mrmep1-FF | GGCTTGCACCCCATTATCACC | |
| Mrmep1-RR | CCATTGCTTGTCTGCCTCTGTTT | ||
| Mrmep2 | Mrmep2-FF | TGATGGAGAAGCGGCAAAATG | |
| Mrmep2-RR | GGCATCACCCCTGTGGTATCTT | ||
| Gallerimycin | gal-F | CTACAGAATCACACGACACT | |
| gal-R | CGAAGACATTGACATCCATT | ||
| gpd | gpd-F | GACTGCCCGCATTGAGAAG | |
| gpd -R | AGATGGAGGAGTTGGTGTTG | ||
| 18S | 18S-F | CGCGCTACACTGAAGGAATC | |
| 18S-R | TTGATTACGTCCCTGCCCTT | ||