Figure 1:

Characterisation of SOX2/PAX6-iNPs. (A) Schematic detailing experimental outline for Studies One and Two. Weekly passaging was commenced at day 31 (Study One) or day 17 (Study Two) post-transfection. (B) – (C) Phase contrast images of fibroblasts (Fib) reprogramming into iNPs over time. Scale bar: 100 μm. (D) – (E) Heat map depicting gene expression in iNPs over time as fold changes relative to fibroblasts. ND: not detected. (F) Expression of neural progenitor and regional markers in p4 SOX2/PAX6-iNPs. Arrowheads indicate some cells with positive staining. Scale bar: 50 μm.