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. 2018 May 30;14(1):34–44. doi: 10.46582/jsrm.1401005

Figure 2:

Figure 2:

Determining the dopaminergic differentiation capacity of SOX2/PAX6-iNPs. (A) Schematic detailing experimental outline for Study Three. (B) Expression of dopaminergic markers in cultures differentiated with Patterning and Maturation media. Arrowheads indicate cells with positive staining. Scale bar: 50 μm. (C) Quantification of TUJ1+, TUJ1+/TH+ and TUJ1+/AADC+ cells in differentiated iNP cultures. Data are presented as mean ± SEM, n = 16 - 32 fields of view from two independent cell lines (number of cells counted per condition ≥ 2519). Unpaired t-tests revealed no effect of SHH/FGF8 patterning on yields of TUJ1+, TH+/TUJ1+ or AADC+/TUJ1+ cells. (D) Quantification of TH+ and AADC+ cells out of TUJ1 in differentiated iNP cultures. Data are presented as mean ± SEM, n = 16 fields of view from two independent cell lines (number of cells counted per condition ≥ 2519). Unpaired t-tests revealed no effect of SHH/FGF8 patterning on yields of TH+ or AADC+ cells.