Figure 3:

Investigating the effect of exposure to patterning molecules during SOX2/PAX6-mediated reprogramming. (A) Schematic detailing experimental outline for Studies Four – Six. SHH: 200 ng/ml SHH-C24II, PUR: 2 μM purmorphamine, CHIR: 0.7 μM CHIR99021. (B) Heat map depicting gene expression in iNPs at p3 as fold changes relative to control SOX2/PAX6-iNPs cultured under standard reprogramming conditions. (C) Phase contrast images of differentiated iNP cultures from Study Six after one week in Patterning media and one week in Maturation media .Scale bar: 50 μm.