Figure 6:

Investigating the effect of adding vmDA transgenes during reprogramming. (A) Schematic detailing experimental outline for Study Nine. Cells were cultured in standard reprogramming medium. (B) Immunocytochemistry at three days post-transfection confirms transfection of plasmids. Scale bar: 50 μm. (C) Heat map depicting gene expression in iNPs as fold changes relative to fibroblasts. (D) Quantification of TUJ1+ and TUJ1+/TH+ cells in differentiated iNP cultures. Data are presented as mean ± SEM, n = 6 – 13 fields of view from one cell line (number of cells counted per condition ≥ 738). An ordinary one-way ANOVA revealed no effect of the transfection factor combination on yields of TUJ1+ or TH+/TUJ1+ cells.