Figure 7:

Investigating the effect of combining vmDA transgenes with patterning molecules during reprogramming.(A) Schematic detailing experimental outline for Study Ten. Standard SHH concentration was 200 ng/ml, low SHH was 20 ng/ml. (B) Heat map depicting gene expression in iNPs as fold changes relative to iNPs generated with the same transgene combinations but reprogrammed in the absence of patterning cues. (C) Quantification of TUJ1+, TUJ1+/TH+ and TUJ1+/AADC+ cells in differentiated iNP cultures. Data are presented as mean ± SEM, n = 6 – 13 fields of view from one cell line (number of cells counted per condition ≥ 1559). Unpaired t-tests were performed to compare the effect of patterning with SHH/CHIR/FGF8 on differentiation yields.