Figure 5.

500 hgPSC colonies were expanded by traditional conditions (i.e., by trypsinization and passaging 1:2) or they were expanded 1:2 in GEM. Comparing two different patients, no marked difference was observed until the second and third passages where GEM-grown hgPSCs grew ~2X faster than conventional growth. By the fourth passage, GEM-grown colonies re-generated close to 4X more colonies when compared to conventional growth and passaging. More importantly, those ~4x more colonies were obtained in almost half the time.