Table 2: Primer Sequences.
CARDIOMYOCYTE PRIMER SEQUENCES (5'-3') | |
SSC GENE CANDIDATES | Abbreviation |
F- AAAGCTTGGCGCAGATGTGA R- TTGCCACGGCATTGGTAAGA |
GPR125 |
F- TTTACCAACTGCCAGCCAGA R- TGTTGCTGCAGTCACACCAT |
GFR1a |
F- GAGAAGCTGTTCCAGATAGTGC R- CTCAGGGTACATGAAATGGTGG |
SSEA-4 |
hESC GENES | Abbreviation |
F- ATGTACAACATGATGGAGACGG R- CCACACCATGAAGGCATTCA |
SOX2 |
F- TTTGCCAAGCTCCTGAAGCA R- AAAGCGGCAGATGGTCGTTT |
OCT3/4 |
F- GAGCATGCAGAAGCGCAGATCAAA R- TATGGCTGATGCTCTGGCAGAAGT |
LIN 28A |
F- TCAGAGACAGAAATACCTCAGC R- AGGAAGAGTAAAGGCTGGGG |
NANOG |
F- TTCAACCTGGCGGACATCAA R- TTCAGCACGAACTTGCCCAT |
KLF-4 |
F- GTATTGCCCTTTGGAGGCAC R- AGGGTCATAACTGGGCACTC |
CD73 |
EARLY CARDIAC GENES | Abbreviation |
F- TTCCAGAACACGATGAGGCA R- ACCTGTGACAGCTTGGTAGA |
ALCAM |
F- AAGGTGTCTAGTAAGACAGCAG R- ATCATTTTGCCTAGCCCACC |
CHAMP |
F- ATGCATTCTGGCGACCATCA R- ACGCCATTCCCAGTACCTTG |
TBX18 |
DIFFERENTIATED CARDIAC GENES | Abbreviation |
F- AGTGGATTGCTCCTTGACGA R- GGGCACGACCTCATCTTCTA |
ANP |
F- ACCCTAGAGCCGAAAAGAAAG R- GCCGCACAGTAATGGTAAGG |
NKX2.5 |
F- AAGATCTCCGCCTCGAGAAA R- GCAGAGATCCTCACTCTCCG |
CTNI |
F- CTTTGATGAGAGACGTCGGG R-CTTCCCACTTTTCCGCTCTG |
CTNT |
F- GGGGACAGTGGTAAAAGCAA R- TCCCTGCGTTCCACTATCTT |
MHC |
F- GAGTTCAAAGAAGCCTTCAGC R- ATCCTTGTTCACCACCCCTT |
MLC2A |
F- GGTGCTGAAGGCTGATTACG R- TTGGAACATGGCCTCTGGAT |
MLC2V |
F-CTGTGGGCTGTTCACCAACT R- GCCGCAACCAACACATAGG |
ISL-1 |
PARACRINE FACTORS | Abbreviation |
F- GGGCAGAATCATCACGAAGT R- TGTTGTGCTGTAGGAAGCTC |
VEGFA |
F- GAGCCTGCGCAATGGAATAA R- ATACCCTGTGGGCTTGTTGA |
IGF-1 |
F- TCAACACTCCAAACTGTGCC R- AGCAAGTGAACTGTGGTCCAT |
SDF-1 |
F- CGACTGGAAGACACGTTTGG R- TTTGGGAGTACGGATGCACT |
CTGF |
F- CACAACAGAGCCAACAGAGTC R- TCCAGGTGGCAGAAGTAGAC |
END-1 |
F- AGAGTGAGTCCAACTCGGTG R- AGGGCAAAGTCCAGGATCTC |
AAMP |
F- GGAGCATATGTGTCTTCAGCTAC R- AAGCTGGCCATTACGTAGTTTTG |
NRG-1 |
TERATOMA GENES | Abbreviation |
F- AAGCGGGATTGTGAGCTGTG R- TGAAGGTCATCATCAGGCGG |
DND 1 |
F- AAAATGGGGGCGGGTTTTTC R-CGCTCCCAGTGACAGTTTCT |
P18 CDKI |
F- CGGCGAGGAGGAGGGAG R- GTCCCTGCGATGGAGATCAG |
P19 CDKI |
GENES EXPRESSED BY ALL EMBRYONIC GERM LAYERS | Abbreviation |
F- TGCCCAGTTTGTTC R- ACATCTCCTCTGCAACAGTGCTCA |
AFP |
F- AGCCATTCCGTAGTGCCATC R- CAGAAGTGTCGCCTCGAAGT |
BMP4 |
F- CAGGAGAAACAGGGCCTACAG R- GCACAGGTGTCTCAAGGGTA |
NES |