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. 2018 May 30;14(1):10–26. doi: 10.46582/jsrm.1401003

Table 3: Comparing expansion rates of primary hgPSCs to expanded hgPSCs grown in GEM.

Passage # 56 yo Patient 1
Number of gPSC colonies
NO Conditional Reprogramming Medium (No GEM)
Time
P0 500 Day 0
P1 380 320 Day 10
P2 280 220 235 215 Day 21
P3 113 163 89 108 123 93 105 113 Day 35
P4 85 94 101 96 58 73 80 93 103 94 76 89 88 91 87 92 Day 60
Total # of
colonies
1,400
Passage # 56yo Patient 1
Number of gPSC colonies
Expanded in Conditional Reprogramming Medium (+ GEM)
Time
P0 500 Day 0
P1 455 476 Day 10
P2 387 358 396 403 Day 15
P3 308 310 303 317 302 287 363 397 Day 21
P4 302 275 280 285 295 283 301 275 267 258 275 285 355 310 365 352 Day 28
Total # of
colonies
4,763
Passage # 31yo Patient 2
Number of gPSC colonies
NO Conditional Reprogramming Medium (No GEM)
Time
P0 500 Day 0
P1 385 380 Day 10
P2 286 222 205 235 Day 21
P3 133 114 113 124 143 127 118 147 Day 35
P4 101 102 88 98 84 88 104 102 110 113 95 101 95 87 115 103 Day 60
Total # of
colonies
1,587
Passage # 31yo Patient 2
Number of gPSC colonies
Expanded in Conditional Reprogramming Medium (+ GEM)
Time
P0 500 Day 0
P1 489 478 Day 10
P2 287 318 337 306 Day 15
P3 221 247 276 285 307 315 287 305 Day 21
P4 205 210 222 230 245 237 275 245 301 275 303 289 275 263 300 268 Day 28
Total # of
colonies
4,143