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. 2018 Jul 6;11:231. doi: 10.3389/fnmol.2018.00231

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Copyright © 2018 Xie, Zhou, Wang, Jiang, Cao, Shao, Wang, Li, Shen and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Knocking down or overexpressing Lgi1 affects the differentiation of OPCs. (A) Cultured OPCs were lentivirally transduced with scramble RNA (NC) or Lgi1-shRNA-GFP (shRNA). Western blots show the expression levels of MBP and CNP were decreased by shRNA (MBP, p = 0.0059; CNP, p = 0.0129; n = 4). As the control for lentivirus infection, Lgi1 expression was analyzed from same samples (p = 0.0037; n = 4). (B) Arrows present GFP+/MBP+ cells. Scale bars, 20 μm. Note the difference in the area and the development of secondary processes in NC and shRNA groups. The right panel shows the quantification of average areas of GFP+/MBP+ cells in NC or shRNA group (p = 0.0095, n = 4). (C) Cultured OPCs were transduced with control lentivirus (NC) or lentiviral plasmid encoding Lgi1 (OE). The levels of MBP and MAG proteins were increased by overexpressing Lgi1 (MBP, p = 0.022; MAG, p = 0.011; n = 4). Meanwhile, increased expression of Lgi1 was confirmed by the expression of Flag that was ligated to lentivirus. (D) White arrows indicate GFP+/MBP+ cells (upper and lower panels) and blue arrows indicate MBP+ cells (lower panel). The results show that the over-expression of Lgi1 (GFP+/MBP+ cells in lower panel) increases the area of OLs. Scale bars, 20 μm. The average areas of GFP+/MBP+ cells in NC or OE group (p = 0.0222, n = 4) was shown in the right panel. *p < 0.05, **p < 0.01.