(A) After treatment with scriptaid for 24 h, HepG2 cells were analyzed using an Annexin V-FITC/PI detection kit. (B) Western blot analysis of apoptosis-associated factors caspase-3, caspase-9, PARP1, and their cleaved forms. (C) Flow cytometry analyses of Hep3B cell apoptosis following treatment with different doses of scriptaid for 24 h. (D) Assessment of caspase-3, caspase-9, PARP1, and corresponding cleaved forms in Hep3B cells treated with scriptaid. Experiments were repeated for at least three times. Error bars: mean + S.D. *, P<0.05; **, P<0.01.