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. 2018 Jul 3;9:1540. doi: 10.3389/fimmu.2018.01540

Figure 2.

Figure 2

Histidine decarboxylase (HDC)-deficient mice show little or no acquired tick resistance. (A) WT and HDC-deficient (KO) mice were infested once or twice with tick larvae. The relative tick repletion was calculated and shown as in Figure 1A (mean ± SEM, n = 3–4). (B–D) WT and KO mice were infested twice with tick larvae. In (B), the number of basophils and mast cells accumulating at the second tick-feeding site on day 2 of the second infestation is shown (mean ± SEM, n = 4 each). In (C), surface expression of CD63 (red histograms) on basophils and mast cells isolated from the second tick-feeding site on day 2 of the second infestation is shown. Black histograms indicate control staining with an isotype-matched antibody. In (D), the frequency (%) of CD63+ cells among basophils and mast cells examined in (C) is summarized (mean ± SEM, n = 3 each). All the data shown are representative of three independent experiments. N.S., not significant; **p < 0.01.