Figure 1.

Mechanical loading, but not breast cancer cell-derived soluble factors, increased MLO-Y4 dendrite formation. (A,C) Fluorescent images of MLOs suggested that fluid shear stress alone increased dendrite formation, but treatment with tumor-conditioned media (TCM) alone did not. (Scale bars = 100 μm; blue = DAPI, red = phalloidin) (B) Image analysis revealed that mechanical loading increased the number of dendrites per MLO, which was achieved via a similar increase in overall dendrite quantity. MLO cell number was not affected by loading. (D) In contrast, no detectable changes in dendrites per MLO, dendrite number, or MLO cell number were observed due to TCM treatment alone. Data are represented as mean + SD. ^*p < 0.05 relative to respective NonLoaded MLO group.