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. 2018 Jul 3;9:352. doi: 10.3389/fendo.2018.00352

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Copyright © 2018 Wang, Sarazin, Kornilowicz and Lynch.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Breast cancer-derived factors modulated MLO-Y4 cell growth during mechanical loading. (A) Fluorescent images of MLOs revealed that mechanical loading promoted dendrite formation independent of the presence of tumor-conditioned media (TCM). (Scale bars = 100 μm; blue = DAPI, red = phalloidin) (B) Mechanical loading increased the number of dendrites per MLO similarly in the absence (upper) and presence (lower) of TCM. In the Control group, loading-induced changes in dendrite/cell occurred due to an increase in overall dendrite quantity whereas in the TCM treatment group, changes occurred due to a decrease in MLO growth with loading. Data are represented as mean + SD. *p < 0.05 relative to respective NonLoaded MLO group.