Skip to main content
. 2018 Jul 3;9:352. doi: 10.3389/fendo.2018.00352

Figure 3.

Figure 3

Mechanically-loaded breast cancer cells altered loading-induced MLO-Y4 dendrite formation. (A) Fluorescent images of MLO cultured with conditioned media collected from NonLoaded MDAs (NonLoaded TCM, upper) and loaded MDAs (Loaded TCM, lower) suggested that mechanically-loaded tumor cells stimulated MLO to form more dendrites. (Scale bars = 100 μm; blue = DAPI, red = phalloidin) (B) Loading increased the number of dendrites per MLO for both N NonLoaded L and Loaded TCM groups. Furthermore, in the N NonLoaded L MLO groups, dendrite per cell was greater when MLOs were treated with Loaded TCM, suggesting that loading both cell types synergistically increased the number of dendrites per MLO. Loading-induced changes to dendrite per cell was achieved primarily via reductions in cell number irrespective of whether TCM came from Loaded or NonLoaded MDAs. Additionally, in NonLoaded MLO groups, MLO quantity was further reduced when treated with Loaded TCM relative to NonLoaded TCM, suggesting that loading applied to both cell types maximizes changes to cell quantity. Data are represented as mean + SD. *p < 0.05 relative to respective NonLoaded (NL) MLO control group, #p < 0.05 relative to NonLoaded MLOs treated with NonLoaded TCM.