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. 2018 Jul 3;9:352. doi: 10.3389/fendo.2018.00352

Figure 4.

Figure 4

Mechanically-loaded breast cancer cells enhanced E11 gene expression and increased the RANKL/OPG ratio in MLO-Y4s. (A) Mechanical loading in MLOs increased their expression of E11, which regulates dendrite formation, which parallels the increased dendrite formation observed histologically and suggests heightened MLO mechanosensivity. (a trend for changes with loading is noted for the NonLoaded TCM group). (B–D) RANKL gene expression was unaffected by loading in both NonLoaded TCM and Loaded TCM groups while OPG expression overall was lower in the Loaded TCM group relative to the NonLoaded TCM group (main effect of TCM treatment; NonLoaded and Loaded MLOs pooled for each TCM treatment), resulting in an overall higher RANKL:OPG ratio in the Loaded TCM group. This results suggests that downstream resorption is heightened when breast cancer cells experience mechanical loading in the skeleton. Data are represented as mean + SD. *p < 0.05 relative to respective NonLoaded MLO control group, ∧p < 0.05 relative to the pooled NonLoaded TCM group (main effect of TCM treatment).