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. 2018 Jul 13;15(1):27–42. doi: 10.3727/105221610x12819686555097

Figure 2.

Figure 2

Adenoviral-mediated transduction efficiency. hMSC were infected using a replication-defective adenoviral vectors carrying either the human LMP3 or the EGFP gene; the adenoviral vector backbone (Adψ5) served as control. (a) EGFP served as a reporter gene, which allowed to assess the transgene expression in vitro by counting EGFP-positive cells under fluorescent invertoscope 24 h after transduction. Scale bar: 10 μm. (b) Transgenic LMP3 expression was assessed in AdLMP3-transduced cells by means of real-time PCR in time course. The GAPDH gene was used as a housekeeping control gene. Expression levels are expressed as mean fold change (FC) over the control, which was calculated according to the ΔΔCt method as reported in the Materials and Methods section.