Figure 4.

Multidimensional scaling of microarray data. Unsupervised multidimensional scaling (MDS) of the expression profiles from LMP3-transduced and control cells was performed in order to assess the segregation efficiency of samples. The high dimensionality of the gene expression data has been reduced to the three dimensions that comprise the greatest variation across the data set. All samples are represented by spheres and positioned in the three-dimensional space, so that the distance between each pair of samples very closely approximates the centered correlation distance for the corresponding sample pair. A clear separation is achieved between adenoviral-transduced and control untransduced cells along one of the three components of the plot. Triangles: AdLMP3-transduced cells; circles: cells transduced with the viral backbone Adψ5; squares: control untransduced cells.