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Real-time PCR validation. The expression of selected transcript from the dataset was analyzed by means of real-time PCR using sequence-specific primers and GAPDH as a housekeeping control gene. Expression levels are expressed as mean fold change over the control, which was calculated according to the ΔΔCt method as reported in the Materials and Methods section. FC1: fold change obtained by means of microarray analysis; FC2: fold change obtained in real-time PCR.
