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. 2018 Jul 13;15(4):153–162. doi: 10.3727/105221612x13372578119571

Figure 5.

Figure 5

Increased expression of neuron-specific marker tubulin βIII in P19 GFPFoxA1 cells, which show an earlier onset of neuronal differentiation through RA treatment. (A, B) P19 GFPFoxA1 cells express tubulin βIII. Total RNA samples were isolated from P19 cells and clone 1, 2, and 3 of P19 GFPFoxA1 cells. The mRNA levels of tubulin βIII, GFPFoxA1, and cyclophilin were measured by RT-PCR Assays (A). Immunostaining of P19 cells or the clone 1 cells was performed with tubulin βIII antibody (B). (C, D) P19 GFPFoxA1 cells showed a more rapid change on the Nanog decrease and the tubulin βIII increase during RA-induced neuronal differentiation. P19 cells and the clone 1 cells were treated with RA according to neural differentiation protocol. Total proteins samples were isolated and the protein levels of GFPFoxA1, tubulin βIII, Nanog, and β-actin were measured by Western blotting (C). The differentiated cell samples induced by RA at day 3 or day 6 were immunostained with tubulin βIII (D).