Figure EV4. Mutation of the TRAF‐interacting motif of caspase‐2 does not disrupt RAIDD binding, while the caspase‐2 prodomain is also important for binding TRAF2.

1. HEK293T cells were co‐transfected with indicated caspase‐2(C320A)‐Flag (wild type or TIM mutant) and HA‐RAIDD constructs. After 48‐h culture for expression, lysates were prepared, followed by anti‐Flag IP and IB.
2. HEK293T cells were transfected with siRNA targeting caspase‐2 3′‐UTR to deplete endogenous caspase‐2. 24 h post‐siRNA transfection, cells were then transfected with indicated Casp2‐mVenus constructs (full‐length C320A, prodomain, or Δ1–169 C320A (Δprodomain)). Casp2‐mVenus variants were immunoprecipitated by GFP‐Trap, followed by IB for co‐immunoprecipitated TRAF2.

Source data are available online for this figure.