Figure 2. CD8‐Lck coupling is a limiting factor for the size of the virtual memory T‐cell compartment.

• A, B
  Percentages of naïve (CD44⁻ CD62L⁺), central memory (CD44⁺ CD62L⁺), and effector/effector memory (CD44⁺ CD62L⁻) (A) and Eomes⁺ Tbet⁺ (B) CD8⁺ LN T cells isolated from CD8WT and CD8.4 mice were determined by flow cytometry. Representative experiments out of seven (A) or five (B) in total.
• C–F
  LN cells and splenocytes were isolated from germ‐free polyclonal CD8WT, CD8.4, and heterozygous CD8^WT/8.4 mice and CD8⁺ T cells were analyzed by flow cytometry. (C) Percentage of CD44⁺ T cells among CD8⁺ LN cells and splenocytes. Mean + SEM. n = 9–14 mice per group from four independent experiments. (D) Percentage of Tbet⁺ Eomes⁺ double‐positive T cells. Mean ± SEM. n = 7–12 mice per group from three independent experiments. (E) Percentage of CD44⁺ CD49d⁻ VM and CD44⁺ CD49d⁺ true memory T cells in the spleen. A representative experiment out of four in total. (F) Absolute numbers of CD8⁺ CD44⁺ CD49d⁻ VM and CD8⁺ CD44⁺ CD49d⁺ true memory T cells in LN and the spleen were quantified. Mean + SEM. n = 9–14 mice from four independent experiments.
• G
  Percentage of CD122^HI CD49d⁻ VM and CD122^LOW CD49⁺ true CM cells among CD8⁺ CD44⁺ CD62L⁺ CM T cells isolated from LN. A representative experiment out of three in total.

Data information: Statistical significance was determined using two‐tailed Mann–Whitney test.Source data are available online for this figure.