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. 2018 May 17;37(14):e96729. doi: 10.15252/embj.201796729

Figure 1. DNA2 binds preferentially to centromeric DNA and is required for centromeric DNA replication.

Figure 1

  1. DNA peaks were identified as described in Materials and Methods. Shown is an alignment of whole‐genome sequencing results to the Hg38 genome. Column 1, Hg38 genome structure (UCSC genome browser); column 2, Hg38 centromere locations; column 3, DNA2 binding sites in HCT‐116 cells identified from ChIP‐seq; column 4, BrdU‐negative DNA from DNA2‐null cells; column 5, common peaks among DNA samples shown in lanes 3 and 4. The chromosome number is listed on the left of the plot.
  2. Fold enrichment of centromeric versus non‐centromeric DNA over input as determined by qPCR of DNA2 and FEN1 ChIP samples. Shown by chromosome (Chr) are the means ± SDs of three biological repeats. See Table EV1 for primers used.
  3. Quantitative analysis of the DNA peaks and bases shown in panel (A).
  4. The most highly enriched repetitive centromeric/paracentromeric motif is shown. Letter size reflects its frequency at the position. P‐value of 1.2e‐88 derived by Fisher's exact test.