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. 2018 Jun 13;37(14):e98321. doi: 10.15252/embj.201798321

Figure 5. Arc‐, slit‐, and ring‐shaped GSDMDN term oligomers imaged at sub‐nanometer resolution.

Figure 5

  • A
    High‐resolution AFM topograph of GSDMDNterm oligomers assembled on SLMs made from POPC, DOPG, DOPS, DOPE, and CL (40:20:10:20:10 molar ratio). After SLMs have been incubated with GSDMD and caspase‐1 for 60 min at 37°C, FD‐based AFM topograph was recorded in buffer solution at room temperature (Materials and Methods).
  • B, C
    GSDMDNterm oligomers shown at higher magnification. Arrows indicate adjacent subunits of oligomers, which show an average distance of 2.3 ± 0.3 nm (mean ± SD, n = 117).
  • D–F
    Average number of subunits per (D) arc‐, (E) slit‐, and (F) ring‐shaped GSDMDNterm oligomer. Black lines are Gaussian fits used to determine mean ± SE of the data, and n indicates the number of oligomers measured.
Data information: The full color range of the topographs corresponds to a vertical scale of 10 nm. Scale bars, 20 nm (A) and 10 nm (B, C).