Figure 5. Arc‐, slit‐, and ring‐shaped GSDMD^{N term} oligomers imaged at sub‐nanometer resolution.

• A
  High‐resolution AFM topograph of GSDMD^Nterm oligomers assembled on SLMs made from POPC, DOPG, DOPS, DOPE, and CL (40:20:10:20:10 molar ratio). After SLMs have been incubated with GSDMD and caspase‐1 for 60 min at 37°C, FD‐based AFM topograph was recorded in buffer solution at room temperature (Materials and Methods).
• B, C
  GSDMD^Nterm oligomers shown at higher magnification. Arrows indicate adjacent subunits of oligomers, which show an average distance of 2.3 ± 0.3 nm (mean ± SD, n = 117).
• D–F
  Average number of subunits per (D) arc‐, (E) slit‐, and (F) ring‐shaped GSDMD^Nterm oligomer. Black lines are Gaussian fits used to determine mean ± SE of the data, and n indicates the number of oligomers measured.

Data information: The full color range of the topographs corresponds to a vertical scale of 10 nm. Scale bars, 20 nm (A) and 10 nm (B, C).