Figure EV5. Analysis of the surface area of the transmembrane pore formed by GSDMD^{N term} oligomers over time.

• A–E
  Time‐lapse AFM topographs showing the assembly and pore formation of GSDMD^Nterm oligomers on SLMs made from POPC, DOPG, DOPS, DOPE, and CL (40:20:10:20:10 molar ratio). Time stamps indicate minutes.
• F–J
  Surface area of transmembrane pores measured in time‐lapse AFM topographs. (F) was taken from (A), (G) from (B), (H) from (C), (I) from (D), and (J) from (E).

Data information: Topographs were extracted from time‐lapse topographs shown in Fig 4 and Appendix Fig S3 and S4. The full color range of the topographs corresponds to a vertical scale of 7.4 nm. Scale bars, 10 nm.