. 2018 Jul 9;62:10.29219/fnr.v62.1447. doi: 10.29219/fnr.v62.1447

Table 3.

Change in the serum metabolites from rats treated with sausage and NDMA compared with control group for different time points and doses

Number	Metabolites	Molecular formula	Mass^a	RT^b	Groups	Fold change^c
Number	Metabolites	Molecular formula	Mass^a	RT^b	Groups	7th week	17th week	25th week	33rd week
1	β-Alanine	C₃H₇NO₂	102	5.17	Sausage diet I	0.50^**	0.36^**	0.50^**	0.52^**
					Sausage diet II	0.77^*	0.67^**	0.61^*	–
					Sausage diet III	0.59^*	0.53^**	0.50^**	0.65^**
					NDMA	0.49^**	0.45^**	0.56^**	–
2	3-aminoisobutyric acid	C₄H₉NO₂	102	5.68	Sausage diet I	0.73^**	0.67^**	0.59^**	–
					Sausage diet II	0.66^**	0.63^**	0.50^*	0.75^**
					Sausage diet III	0.43^**	0.49^**	0.39^**	0.57^**
					NDMA	0.23^**	0.25^**	–	0.24
3	Aminooxyacetic acid	_	160	6.12	Sausage diet I	–	0.67^**	–	–
					Sausage diet II	–	–	0.71^*	–
					Sausage diet III	–	–	0.64^*	0.76^*
					NDMA	0.75^*	0.73^*	0.65^*	0.48^**
4	D-alanyl-D-alanine	C₆H₁₂N₂O₃	96	7.51	Sausage diet I	–	0.77^*	–	0.54^*
					Sausage diet II	–	–	–	–
					Sausage diet III	–	–	0.72^*	0.83^*
					NDMA	–	0.81^*	0.62^*	0.63^*
5	Pelargonic acid	C₉H₁₈O₂	118	9.38	Sausage diet I	–	0.68^*	–	–
					Sausage diet II	0.68^*	–	–	0.79^*
					Sausage diet III	–	0.72^*	–	0.68^*
					NDMA	0.69^*	0.49^*	0.71^*	0.66^*
6	Putrescine	C₄H₁₂N₂	86	11.54	Sausage diet I	3.95^**	2.81^**	4.44^**	3.73^**
					Sausage diet II	2.19^**	1.68^**	0.00^**	1.29^*
					Sausage diet III	2.79^**	2.26^**	1.48^**	1.60^*
					NDMA	3.76^**	2.93^**	1.92^**	2.04^**
7	α-Ketoglutarate	C₅H₆O₅	203	13.51	Sausage diet I	–	–	–	1.42^*
					Sausage diet II	–	0.70^**	–	–
					Sausage diet III	–	–	1.30^*	0.58^**
					NDMA	–	1.03^*	1.83^*	2.37^**
8	Phenylalanine	C₉H₁₁NO₂	192	14.54	Sausage diet I	–	–	0.84^*	–
					Sausage diet II	–	0.86^**	0.81^*	1.13^*
					Sausage diet III	–	–	0.85^*	1.12^*
					NDMA	–	1.23^**	1.35^**	1.75^**
9	Ethanolamine phosphate	C₂H₈NO₄P	299	15.22	Sausage diet I	2.47^*	3.17^*	3.08^*	3.66^*
					Sausage diet II	1.97^*	1.37^*	–	2.15^**
					Sausage diet III	–	2.28^**	2.11^**	2.53^*
					NDMA	3.63^**	2.95^**	2.86^**	3.97^**
10	Taurine	C₂H₇NO₃S	188	15.34	Sausage diet I	2.07^**	2.66^**	1.58^*	1.51^*
					Sausage diet II	1.99^**	1.60^**	1.55^*	1.38^*
					Sausage diet III	2.86^**	1.73^*	2.11^**	1.55^*
					NDMA	2.81^**	2.35^**	2.94^**	2.21^**
11	Tyrosine	C₉H₁₁NO₃	218	20.86	Sausage diet I	–	0.84^*	0.75^*	0.83^*
					Sausage diet II	0.83^*	0.79^*	0.81^*	0.76^**
					Sausage diet III	0.86^*	0.79^*	0.77^*	0.81^*
					NDMA	–	–	1.25^*	2.14^**
12	Palmitic acid	C₁₆H₃₂O₂	118	22.89	Sausage diet I	–	0.64^**	0.66^*	0.62^**
					Sausage diet II	–	0.76^*	0.76^*	0.72^*
					Sausage diet III	0.63^**	0.71^*	0.60^**	0.72^*
					NDMA	0.58^**	0.71^*	0.67^*	0.73^*
13	Linoleic acid	C₄H₄O₅	82	25.19	Sausage diet I	–	0.52^**	0.31^**	0.32^**
					Sausage diet II	–	0.59^**	0.47^**	0.38^**
					Sausage diet III	0.45^**	0.42^**	0.31^**	0.35^**
					NDMA	0.69^*	–	–	–

Calculation mass;

RT: retention time (minutes);

significantly increased or decreased when compared with the control group at p < 0.05;

^**

significantly increased or decreased when compared with the control group at p < 0.001;

relative concentration of treated rats to the control at the same time. NDMA, N-nitrosodimethylamine; CON, control.