Table 1.
Relative proteins expression and apoptosis in control and treated podocytes
| Ctl | AII (1 μM) | AII/Los (1 μM) | AII/SB (0.1 μM) | n | |
|---|---|---|---|---|---|
| Protein | |||||
| GRP 78 | 1.00 ± 0.00 | 1.71 ± 0. 15a | 1.05 ± 0.01d | – | 4 |
| peIF2-α | 1.00 ± 0.00 | 1.87 ± 0. 21b | 1.12 ± 0.09f | – | 4 |
| pPKC-δ | 1.00 ± 0.00 | 1.59 ± 0.04a | 1.05 ± 0.10d | – | 5 |
| pP38MAPK | 1.00 ± 0.00 | 1.70 ± 0.03a | 0.94 ± 0.06d | – | 4 |
| Total Bax | 1.00 ± 0.00 | 1.64 ± 0.11a | – | 0.95 ± 0.03d | 4 |
| Total Bcl-2 | 1.00 ± 0.00 | 1.19 ± 0.07 | – | 1.04 ± 0.03 | 4 |
| Bax/Bcl-2 ratio | 1.00 ± 0.00 | 1.39 ± 0.08a | – | 0.90 ± 0.01d | 4 |
| Cell Viability % | |||||
| Viable | 90.93 ± 0.70 | 87.34 ± 0.59c | – | 92.65 ± 0.73d | 5–6 |
| Early apoptosis | 0.62 ± 0.06 | 0.80 ± 0.07 | – | 0.43 ± 0.03f | 5–6 |
| Late Apoptosis | 4.99 ± 0.28 | 6.99 ± 0.50b | – | 4.61 ± 0.30e | 5–6 |
| Necrosis | 3.43 ± 0.47 | 3.97 ± 0.36 | – | 2.48 ± 0.41 | 5–6 |
Values are means ± SEM. n, number of experiments. ap < 0.001, bp < 0.01 and cp < 0.01 versus control (Ctl); dp < 0.001, ep < 0.01 and fp < 0.05 versus angiotensin II (Ang II); Los losartan, SB SB203580; p phosphorylated protein expression