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. 2018 Jul 13;49:59. doi: 10.1186/s13567-018-0555-5

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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qPCR analysis of TsSP transcription level at different stages of T. spiralis. The TsSP mRNA from AW at 3 and 6 dpi, and NBL, ML and IIL were amplified by qPCR. The TsSP mRNA transcription levels were calculated via the Ct (2^−ΔΔCt) method. G3PDH was utilized as a housekeeping gene control. TsSP transcription level was not statistically different among different stages of T. spiralis (P = 0.05).