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. 2018 Jul 13;49:59. doi: 10.1186/s13567-018-0555-5

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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ADCC assays of T. spiralis NBL (A–C) and ML (D–F). A, D The cytotoxicity was anti-rTsSP antibody dose-dependent. B, E The cytotoxicity had an increasing trend with prolonged culture time. C, F ADCC assay with different serum. When 1:100 dilutions of different serum was used and cultured for 72 h, the ADCC with anti-rTsSP serum caused the evident death of NBL (C) and ML (F), compared with the larvae incubated by normal mouse serum (pre-immune serum). Asterisks show a statistically prominent difference (P < 0.01) in comparison with normal serum.