Fig. 6.

Expression profiles of nitrogen (N) starvation-responsive microRNAs in rice shoots and roots. a and b Differential expression of rice microRNAs (miRNAs) between 0 day (-N_0d) and 7 day (-N_7d) of N starvation in rice shoots (a) and roots (b). Fold changes of miRNAs with > 2-fold up- or down-regulation are represented using a log₂ scale. c Northern blot analysis of microRNAs in N-starved rice shoots and roots. d Heatmap showing the expression level of osa-miR169 family and their OsNF-YA target genes in N-starved rice shoots and roots. e Relative expression level of four OsNF-YA genes measured by RNA-Seq and qRT-PCR in N-starved (-N) rice shoots and roots