Fig. 6.

Mutant FBXW7 TVH clone 2 recognizes naturally processed and presented mFBXW7 antigen (a) FBXW7 TVH clone 2 was co-cultured with LCLs loaded with decreasing concentrations of FBXW7 mutant or wildtype peptide at a 1:1 ratio. Reactivity was determined by IFN-γ intracellular cytokine staining. (n = 3) (b) FBXW7 TVH clone 2 was co-cultured with mFBXW7⁺ LCL or mock-transduced LCL at a 1:1 ratio. Reactivity was determined by IFN-γ intracellular cytokine staining. (n = 3) (c) FBXW7 TVH clone 2 was co-cultured with HLA-A*11:01⁺ CML-T1 or HLA-A*11:01⁺ RPMI8402, with or without IFN-γ pre-treatment, at a 1:1 ratio. Reactivity was determined by IFN-γ intracellular cytokine staining. (n = 2) (d) HLA-A*11:01⁺ CML-T1, HLA-A*11:01⁺ RPMI8402 or HLA-A*11:01⁺ LCLs were infected with an EBNA3B MVA encoding the EBV derived epitope IVT, or an empty control MVA. IVT-specific CD8⁺ T cells were co-cultured with EBNA3B MVA infected target cells at a 1:1 ratio. Reactivity was determined by IFN-γ intracellular cytokine staining. (n = 2)