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. 2018 Mar 19;32(8):4612–4623. doi: 10.1096/fj.201701535RR

Figure 7.

Figure 7

Decreased TRPV4 single-channel activity and impaired responses to GSK1016790A in human ADPKD cells. A) Representative continuous current trace from cell-attached patches monitoring TRPV4 activity in NHK (top) and ADPKD (bottom) cells in control and during the application of 30 nM GSK1016790A (shown with respective bars on the top of each trace). Patches had the holding potential of Vh = −Vp = −60 mV. Inward currents are downward. Dashed lines indicate the respective current state with a c denoting the closed state. B) Summary graph of TRPV4 activity (NPo) in control and in response to GSK1016790A from patch-clamp experiments in NHK and ADPKD cells that contained at least 1 active channel and similar to those shown in panel A. C) Pie charts showing the frequency of observing patches with active TRPV4 channels in NHK and ADPKD cells. Asterisk denotes significant decrease vs. respective NHK. *P < 0.05.