Figure 8.

Diminished TRPV4 glycosylation in human ADPKD cells. A) Representative Western blot of whole-cell lysates from primary cultured NHK and ADPKD cells from different kidneys. Lysates were probed with anti-TRPV4 and anti–β-actin Abs. B–D) Summary graphs comparing total (B), upper glycosylated (C), and bottom nonglycosylated (D) forms of TRPV4 from Western blots similar to that shown in panel A. Intensities of the respective TRPV4-reporting reporting bands were normalized to those of the corresponding actin bands. g, glycosylated form of TRPV4. Asterisk denotes significant difference vs. NHK. *P < 0.05.