Skip to main content
. 2018 Jul 10;6:e5202. doi: 10.7717/peerj.5202

Figure 2. dmeFR PCR analysis.

Figure 2

Induction of dmeF and dmeR of wild type or dmeR mutant by various metals examined through quantitative real-time PCR analysis. Wild type and dmeR mutants of S. meliloti CCNWSX0020 strains at OD600 of 1.0 were incubated with 0.5 mM CuSO4, ZnCl2, Pb(NO3)2, NiCl2, 0.2 mM CdCl2 and 0.3 mM CoCl2 for 30 min. The fold changes in dmeF and demR expression are expressed relative to the untreated control. Samples were then processed for qPCR analysis and normalized against the ribosomal 16 S rRNA. Error bars represent standard deviations of three biological repeats.