Table 6. Linear, interactive and quadratic terms for the effects of pH, temperature and surfactants concentration on biofilm formation by B. infantis DSM20088 and L. reuteri DSM20016.

	Sodium dodecyl sulphate (SDS)		Polysorbate 80 (PS80)
	B. infantis	L. reuteri	B. infantis	L. reuteri
Linear terms
[pH]	nsa	3.090	−2.289	−3.339
[temperature]	3.259	ns	7.622	8.321
[surfactant]	−2.880	−2.420	ns	ns
Interactive terms
[pH]*[temperature]	ns	ns	ns	ns
[pH]*[surfactant]	ns	ns	ns	ns
[surfactant]*[temperature]	ns	ns	ns	ns
Quadratic terms
[pH]2	ns	ns	ns	ns
[temperature]2	−2.435	ns	−7.228	−8.499
[surfactant]2	ns	ns	ns	ns
Rb	0.902	0.977	0.998	0.885
SEc	1.008	1.022	0.977	1.039

Note:

Both an anionic surfactant (SDS) and a non-ionic surfactant (PS80) were used.

^aNot significant;

^bR, regression coefficient;

^cSE, standard error.