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. Author manuscript; available in PMC: 2019 Apr 24.
Published in final edited form as: Cell Rep. 2018 Apr 24;23(4):1192–1204. doi: 10.1016/j.celrep.2018.03.112

Figure 1. Disruption of HOIL-1L UBL Results in Embryonic Lethality.

Figure 1.

(A) Number of offspring of each genotype resulting from crosses of HOIL-1L+/null” mice.

(B) Numbers of embryos obtained at each embryonic stage (E9.5, 10.5, 11.5, and 12.5) from crosses of HOIL-1L+/null” mice.

(C) Representative gross appearance of HOIL-1L-null and WT littermate on embryonic day 11.5 (E11.5).

(D) Immunoblot analyses of lysates of MEFs from mice of the indicated genotypes.

(E) Indicated MEFs were stimulated with TNF-α (10 ng/ml), and cell viability was continuously measured on a real-time cell analyzer (RTCA) (means ± SEM, n = 3).

(F) Immunoblot analysis of lysates of MEFs from mice of the indicated genotypes. ns, non-specific band.

(G) Immunoblot analysis of lysates of HOIL-1L-null MEFs reconstituted with HOIL-1L UBL.

(H) Cell viability of the indicated MEFs was measured as described in Figure 1E (means ± SEM, n = 4).

See also Figure S1.