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Primer sequences for reverse transcription quantitative polymerase chain reaction
| Gene | Primer sequence (5′–3′) |
|---|---|
| SPINT1-AS1 | |
| Reverse transcription | TCAAGGTGTCAGCCAGCACT |
| Forward | GCCCTGGAGGATGAGAG |
| Reverse | CAGATGCTGTTGGCTAAAGA |
| SPINT1 mRNA | |
| Reverse transcription | CCGTAACAACCACCATAGGT |
| Forward | ACCTGTCAGCCCACCCAGTT |
| Reverse | CCACGCAGTGCCCTTTGTCA |
| GAPDH | |
| Forward | TGCACCACCAACTGCTTAGC |
| Reverse | GGCATGGACTGTGGTCATGAG |
| UBC | |
| Forward | AGTAGTCCCTTCTCGGCGAT |
| Reverse | GACGATCACAGCGATCCACA |
