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. 2018 Jul 10;11:3959–3968. doi: 10.2147/OTT.S156143

Figure 2.

Figure 2

SIRT4 suppresses GC cell proliferation. (A) MKN-45 and HGC-27 were transfected with vector or SIRT4, scramble siRNA (SCR), or SIRT4 siRNA (siSIRT4). After transfection for 48 h, the mRNA level of SIRT4 was determined using qRT-PCR. SIRT4 vs vector and siSIRT4 vs SCR, *p < 0.05. (B) MKN-45 and HGC-27 cells were transfected with vector or SIRT4, scramble siRNA (SCR), or SIRT4 siRNA (siSIRT4). After transfection for 48 h, the protein level of SIRT4 was determined using Western blotting. (C) The effect of SIRT4 on cell proliferation was analyzed by CCK-8 assay. SIRT4 vs vector and siSIRT4 vs SCR, *p < 0.05. (D) Colony formation assay was performed to detect the effect of SIRT4 on cell proliferation. SIRT4 vs vector and siSIRT4 vs SCR, *p < 0.05. siSIRT4#1: 5′-AAGAGTTACAGCGCTTCATCACC-3′; siSIRT4#2: 5′-CGATTGCAATACTGAACATTGG-3′.

Abbreviations: CCK-8, Cell Counting Kit-8; GC, gastric cancer; OD, optical density; qRT-PCR, quantitative real-time polymerase chain reaction.