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. 2018 Jan 30;46(7):3764–3773. doi: 10.1093/nar/gky052

Figure 2.

Figure 2.

Interactions of Cwc23 with the C-terminus of Ntr1. (A) Maps of Ntr1, Ntr2 and Cwc23 proteins showing conserved motifs of the proteins and Ntr1 domains for deletion analysis. Regions responsible for Ntr1 interactions with Prp43 and Ntr2 are shown above the map. G, G-patch; CC, coiled coil; J, J-domain; CT, C-terminal fragment. (B) Two-hybrid assays for interactions of Cwc23 with NTR components. (C) Two-hybrid assays for interactions of Cwc23 with Ntr1 deletion mutants. (D) Pull-down of GST-fused Ntr1CT by His-Cwc23 probed with anti-GST and anti-Cwc23 antibodies. I, input; P, precipitates.