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. 2018 Jan 30;46(7):3764–3773. doi: 10.1093/nar/gky052

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Metabolic depletion of Cwc23 resulted in co-depletion of Ntr1 and Ntr2. (A) Splicing assays were performed in wild-type extracts (lane 1) or extracts metabolically-depleted of Ntr2 (lanes 2 and 3) or Cwc23 (lanes 4 and 5), without (lanes 1, 2 and 4) or with (lanes 3 and 5) supplementation of purified NTR complex. (B) Western blotting of wild-type (lane 4) or Ntr2-metabolically-depleted extracts (lane 5) or untreated (lane 3), mock-treated (lane1) or anti-Cwc23 antibody-treated (lane 2) Cwc23-metabolically-depleted extracts. M, mock-treated; Depl, depletion.