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. 2018 Jun 26;9(49):29047–29063. doi: 10.18632/oncotarget.25540

Figure 3. TNFα stimulation of OSCC cells leads to increased matrix degradation and spheroid volume.

Figure 3

(A) Representative confocal images of UMSCC1 formed spheroids at Day 0, 7, and 14. Spheroid volume was revealed through bright-field imaging and Geltrex degradation through 505 nm excitation of BSA-Green degradation marker. Scale bar, 100 μm. (B) Spheroid volume over a period of 17 days in the presence or absence of TNFα (10 ng/mL) or GM6001 (25 µM). (C) Geltrex degradation volume over a period of 17 days in the presence or absence of TNFα (10 ng/mL) or GM6001 (25 µM). Volumes are presented as mean per spheroid ± SEM. Two-way ANOVA followed by Bonferroni post hoc test: a, P < 0.01 TNFα vs. unstimulated; b, P < 0.01 for GM6001 vs. unstimulated; n = 3.