Figure 5. Hypermethylation profiles for the circRNA host genes in primary tumors and their association with transcript down-regulation.

(A) Percentage of TUSC3, ATRNL1, POMT1 and SAMD4A promoter CpG island hypermethylated cases in the TCGA panel of primary tumors by tissue type. Barr codes: Adrenocortical carcinoma (ACC), Bladder urothelial carcinoma (BLCA), Breast invasive carcinoma (BRCA), Cervical and endocervical cancers (CESC), Cholangiocarcinoma (CHOL), Colon adenocarcinoma (COAD), Lymphoid Neoplasm Diffuse Large B-cell Lymphoma (DLBC), Esophageal carcinoma (ESCA), FFPE Pilot Phase II (FPPP), Glioblastoma multiforme (GBM), Glioma (GBM/LGG), Head and Neck squamous cell carcinoma (HNSC), Kidney Chromophobe (KICH), Kidney renal clear cell carcinoma (KIRC), Kidney renal papillary cell carcinoma (KIRP), Acute Myeloid Leukemia (LAML), Brain Lower Grade Glioma (LGG), Liver hepatocellular carcinoma (LIHC), Lung adenocarcinoma (LUAD), Lung squamous cell carcinoma (LUSC), Mesothelioma (MESO), Ovarian serous cystadenocarcinoma (OV), Pancreatic adenocarcinoma (PAAD), Pheochromocytoma and Paraganglioma (PCPG), Prostate adenocarcinoma (PRAD), Rectum adenocarcinoma (READ), Sarcoma (SARC), Skin Cutaneous Melanoma (SKCM), Stomach adenocarcinoma (STAD), Testicular Germ Cell Tumors (TGCT), Thyroid carcinoma (THCA), Thymoma (THYM), Uterine Corpus Endometrial Carcinoma (UCEC), Uterine Carcinosarcoma (UCS) and Uveal Melanoma (UVM). (B) Promoter CpG island hypermethylation of the genes studied is significantly associated with downregulation of the corresponding transcript in the TCGA panel of primary tumors. The box plots display the distribution of the expression values with the central solid line representing the median and the limits of the box, the upper and lower quartiles. The whiskers represent the minimum and maximum values excluding outliers (<1.5 × the interquartile range).