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. 2018 Jun 26;9(49):29146–29161. doi: 10.18632/oncotarget.25635

Figure 4. Δ133p53 inhibits TAp63 and TAp73 and Δ133TP53 RNA expression is inversely correlated with TP63 associated genes in ER- wtTP53 tumours.

Figure 4

(A) Δ133p53 was immunoprecipitated with the p53 conformation specific antibody pAb240 and immunoblotted with the same antibody. (B-C) TAp63 and TAp73 were immunoprecipated and immunoblotted with pAB240. (D) An example of the colony formation assay carried out in H1299 cells, co-transfected with a TAp63 expression construct and increasing amounts of a Δ133p53 expressing plasmid, along with the CMVNeo plasmid. (E) Quantification of G418 resistant colonies after transfection with TAp63 and Δ133p53 (left graph) and TAp73 and Δ133p53 (right graph). The bars represent the mean and error bars are ± S.D. of 8 biological replicates. (F) Association of the 191 genes associated with TP63 RNA expression in ER- wtTP53 tumours from TCGA, GSE61725 and GSE3494 and with Δ133TP53 RNA expression in ER- wtTP53 tumours from GSE61725.