Table 1.
Saccharomyces cerevisiae strains used in this study.
| Strain | Relevant genotype | Reference |
|---|---|---|
| CEN.PK 113–7D | MATa MAL2–8c SUC2 | Nijkamp et al.2012 |
| IMX080 | MATa ura3–52 his3–1 leu2–3112 MAL2–8c SUC2 glk1::Sphis5, hxk1::KlLEU2 | Solis-Escalante et al.2015 |
| IMX486 | MATa ura3–52 his3–1 leu2–3112 MAL2–8c SUC2 glk1::Sphis5, hxk1::KlLEU2 gal1::cas9-amdS | This study |
| IMX604 | MATa ura3–52 his3–1 leu2–3112 MAL2–8c SUC2 glk1::Sphis5, hxk1::KlLEU2 gal1::cas9- amdS gre3::pTDH3_RPE1 pPGK1_TKL1, pTEF1_TAL1 pPGI1_NQM1 pTPI1_RKI1 pPYK1_TKL2 | This study |
| IMX658 | MATa ura3–52 his3–1 leu2–3112 MAL2–8c SUC2 glk1::Sphis5, hxk1::KlLEU2 gal1::cas9- amdS gre3::pTDH3_RPE1 pPGK1_TKL1, pTEF1_TAL1 pPGI1_NQM1 pTPI1_RKI1 pPYK1_TKL2 gal80::(pTPI_araA_tCYC)*9 pPYK-araB-tPGI1 pPGK-araD-tTDH3 | This study |
| IMX660 | MATa ura3–52 his3–1 leu2–3112 MAL2–8c SUC2 glk1::Sphis5, hxk1::KlLEU2 gal1::cas9- amdS gre3::pTDH3_RPE1 pPGK1_TKL1, pTEF1_TAL1 pPGI1_NQM1 pTPI1_RKI1 pPYK1_TKL2 gal80::(pTPI_araA_tCYC)*9 pPYK-araB-tPGI1 pPGK-araD-tTDH3 hxk2::KlURA3 | This study |
| IMX728 | MATa ura3–52 his3–1 leu2–3112 MAL2–8c SUC2 glk1::Sphis5, hxk1::KlLEU2 gal1::cas9- amdS gre3::pTDH3_RPE1 pPGK1_TKL1, pTEF1_TAL1 pPGI1_NQM1 pTPI1_RKI1 pPYK1_TKL2 gal80::(pTPI_araA_tCYC)*9 pPYK-araB-tPGI1 pPGK-araD-tTDH3 hxk2:: PcaraT | This study |
| IMX1106 | MATa ura3–52 his3–1 leu2–3112 MAL2–8c SUC2 glk1::Sphis5, hxk1::KlLEU2 gal1::cas9- amdS gre3::pTDH3_RPE1 pPGK1_TKL1, pTEF1_TAL1 pPGI1_NQM1 pTPI1_RKI1 pPYK1_TKL2 gal80::(pTPI_araA_tCYC)*9 pPYK-araB-tPGI1 pPGK-araD-tTDH3 hxk2::KlURA3 gal2::gal2N376I | This study |
| IMX1386 | MATa ura3–52 his3–1 leu2–3112 MAL2–8c SUC2 glk1::Sphis5, hxk1::KlLEU2 gal1::cas9- amdS gre3::pTDH3_RPE1 pPGK1_TKL1, pTEF1_TAL1 pPGI1_NQM1 pTPI1_RKI1 pPYK1_TKL2 gal80::(pTPI_araA_tCYC)*9 pPYK-araB-tPGI1 pPGK-araD-tTDH3 hxk2::KlURA3 CAN1::pGAL2_GAL2_tGAL2 | This study |
| IMS0514 | IMX728 that has undergone laboratory evolution on a mixture of arabinose and glucose under aerobic conditions | This study |
| IMS0520 | IMX728 that has undergone laboratory evolution on mixture of arabinose, glucose and xylose under anaerobic conditions | This study |
| IMS0521 | IMX728 that has undergone laboratory evolution on mixture of arabinose, glucose and xylose under anaerobic conditions | This study |
| IMS0522 | IMX728 that has undergone laboratory evolution on mixture of arabinose, glucose and xylose under anaerobic conditions | This study |
| IMS0523 | IMX728 that has undergone laboratory evolution on mixture of arabinose, glucose and xylose under anaerobic conditions | This study |
| IMW088 | As IMS0522; PcAraTΔ | This study |
| IMW091 | As IMS0522; gal2I89T | This study |
| DS68616 | Mat a, ura3–52, leu2–112, gre3::loxP, loxP-Ptpi:TAL1, loxP-Ptpi::RKI1, loxP-Ptpi-TKL1, loxP-Ptpi-RPE1, delta::Padh1XKS1Tcyc1-LEU2, delta::URA3-Ptpi-xylA-Tcyc1 | DSM, The Netherlands |
| DS68625 | DS68616, his3::loxP, hxt2::loxP-kanMX-loxP, hxt367::loxP-hphMX-loxP, hxt145::loxP-natMX-loxP, gal2::loxP-zeoMX-loxP | Nijland et al.2014 |
| DS68625-Gal2 | DS68616, his3::loxP, hxt2::loxP-kanMX-loxP, hxt367::loxP-hphMX-loxP, hxt145::loxP-natMX-loxP, gal2::loxP-zeoMX-loxP | This study |
| DS68625-Gal2N376I | DS68616, his3::loxP, hxt2::loxP-kanMX-loxP, hxt367::loxP-hphMX-loxP, hxt145::loxP-natMX-loxP, gal2::loxP-zeoMX-loxP | This study |
| DS68625-Gal2N376S | DS68616, his3::loxP, hxt2::loxP-kanMX-loxP, hxt367::loxP-hphMX-loxP, hxt145::loxP-natMX-loxP, gal2::loxP-zeoMX-loxP | This study |
| DS68625-Gal2N376T | DS68616, his3::loxP, hxt2::loxP-kanMX-loxP, hxt367::loxP-hphMX-loxP, hxt145::loxP-natMX-loxP, gal2::loxP-zeoMX-loxP | This study |
| DS68625-Gal2N376T/T89I | DS68616, his3::loxP, hxt2::loxP-kanMX-loxP, hxt367::loxP-hphMX-loxP, hxt145::loxP-natMX-loxP, gal2::loxP-zeoMX-loxP | This study |
| DS68625-Gal2T89I | DS68616, his3::loxP, hxt2::loxP-kanMX-loxP, hxt367::loxP-hphMX-loxP, hxt145::loxP-natMX-loxP, gal2::loxP-zeoMX-loxP | This study |
| DS68625-mcs | DS68616, his3::loxP, hxt2::loxP-kanMX-loxP, hxt367::loxP-hphMX-loxP, hxt145::loxP-natMX-loxP, gal2::loxP-zeoMX-loxP | This study |