Table 3.
Estimated copy numbers of the heterologously expressed Lactobacillus plantarum genes araA, araB and araD and Penicillium chrysogenum PcaraT in the evolved, anaerobically growing and glucose-tolerant ‘arabinose specialist’ strains S. cerevisiae IMS0520-IMS0523, obtained from two independent evolution experiments in SBRs (1 and 2), and in the parental strain IMX728. Copy numbers were estimated by comparing gene read depths to the average of all chromosomes.
| Reference | Reactor 1 | Reactor 2 | |||
|---|---|---|---|---|---|
| Gene | IMX728 | IMS0520 | IMS0521 | IMS0522 | IMS0523 |
| araA | 17.5 | 8.7 | 19.3 | 19.5 | 16.4 |
| araB | 0.8 | 0.9 | 3.1 | 2.2 | 1.2 |
| araD | 0.9 | 1.0 | 1.2 | 1.0 | 1.1 |
| PcaraT | 0.9 | 1.1 | 1.0 | 1.1 | 1.2 |
| 1 Excluded chromosomes: | 3, 10, 12, 16 | 2, 8, 12 | 12 | 12, 16 | |
1Chromosomes excluded from the average read depth, based on duplication of chromosomal regions identified with the Magnolya algorithm (Nijkamp et al.2012).