FIGURE 5.

Variability of the proinflammatory cytokine IL-6 relative expression in different colon segments. mRNA relative expression of IL-6 in the 3 segments of the colon, proximal (A, D), middle (B, E), and distal (C, F). qRT-PCR was performed by the 1-step protocol, and data are expressed as the relative expression calculated using the Pfaffl method¹⁶ and β-actin (A–C) or Eef2 (D–F) as reference genes. Results are expressed as means ± sd; n = 5 mice per group. Mann-Whitney, *P < 0.05, **P < 0.01.