TABLE 2.

Descriptive performance of 1-step dilution curves for β-actin, Eef2, and IL-6 using RNA from control mice (C_t values)

Primer	RNA, ng	Minimum	Maximum	Median	Mean	sd
β-Actin	200	16.79	16.86	16.82	16.82	0.035
	20	20.21	20.54	20.48	20.41	0.176
	2	23.67	23.86	23.68	23.74	0.107
	0.2	26.95	27.46	27.33	27.25	0.265
Eef2	200	17.32	17.96	17.61	17.63	0.320
	20	21.15	21.43	21.36	21.31	0.145
	2	24.72	24.98	24.85	24.85	0.130
	0.2	29.19	29.21	29.19	29.20	0.111
IL-6	200	27.55	28.14	27.93	27.87	0.299
	20	31.59	32.03	31.62	31.75	0.246
	2	34.54	35.23	34.95	34.91	0.347
	0.2	38.76	39.91	39.20	39.29	0.580