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. 2018 Mar 22;99(1):212–224. doi: 10.1093/biolre/ioy070

Figure 4.

Figure 4.

(A) Comparative expression of transcription factors that play roles in TE emergence in undifferentiated human ESC (ESCu; blue bars) and differentiated ESC (ESCd; red bars). Transcript levels are shown in FPKM (Fragments Per Kilobase of transcript per Million mapped reads). RNAseq data are from Yabe et al. [80]. (B) Fold-change differences in relative expressions of known trophoblast gene markers in STB from ESCd (>70 μm size fraction from BAP-differentiated ESC) and from STB generated from placenta at term (PHTd). Data are from Yabe et al. [80]. Gray horizontal bars indicate where differences in gene expression are not significant (q > 0.05). All other values differ between the two types of cell. (C) Immunofluorescent detection of CGA (green) and GATA2 (red) in a human iPSC cell line cultured in the presence of BAP for 6 days. The image represents cells in a part of the iPSC colony that include STB areas (see live images in Supplementary Figure S1, day 6) [27]. Nuclear materials are stained with DAPI (blue). The scale bar represents 200 μm.