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. 2018 May 8;77(8):665–672. doi: 10.1093/jnen/nly042

FIGURE 3.

FIGURE 3.

Expression of mitochondrial respiratory chain proteins in Sdha+/- rats. Western blot analysis of gastrocnemius muscle did not yield any significant differences in mitochondrial respiratory chain proteins (C-I, C-II-70, C-III-Core 2, C-IV Subunit IV, and C-V Alpha) between 6-week wild-type (n = 4) and 6 week Sdha+/- (n = 4) or 6-month wild-type (n = 4) and 6-month Sdha+/- (n = 4) rats. C-IV is shown on a different panel because its optimal exposure time was much lower. GAPDH is shown in a different panel as it required stripping/reprobing to evaluate. (A) Western blots of 6-week wild-type, 6-week Sdha+/-, 6-month wild-type, and 6-month Sdha+/- rats (n = 4) as representative sample of gastrocnemius muscle that was quantified. (B) Quantified mitochondrial respiratory chain protein expression for 6-week-old wild type (n = 6), 6-week Sdha+/- (n = 5), 6-month-wild type (n = 8), and 6-month Sdha+/- (n = 8) rats were normalized to GAPDH and graphed. Data were analyzed using two-way ANOVA.