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. Author manuscript; available in PMC: 2018 Jul 13.
Published in final edited form as: Exp Hematol. 2017 Apr 27;51:17–24. doi: 10.1016/j.exphem.2017.04.003

Figure 1.

Figure 1

Expression of CD25 on CD34+/CD38− LSCs in patients with CML and on KU812 cells. (A) Primary CML cells were stained with fluorochrome-labeled monoclonal antibodies against CD34, CD38, CD45, CD25, CD122, and CD132. LSCs were defined as CD45+/CD34+/CD38− cells. As assessed by multicolor flow cytometry, LSCs were found to co-express CD25 (left panel), but did not express CD122 (middle panel) or CD132 (right panel) (red histograms). The isotype-matched control antibody is also shown (black open histograms). (B) The Ph+ cell line KU812 was stained with antibodies against CD25, CD122, and CD132 (blue histograms). It can be seen that KU812 cells also express CD25 and do not express substantial amounts of CD122 or CD132.